Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 405

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:20,000

Immunohistochemistry (IHC)

1:50-1:2,000

Immunocytochemistry (ICC/IF)

1:50-1:2,000

Flow Cytometry (Flow)

1:25 - 1:100

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

DyLight™ 405 DyLight™ 405 DyLight™ 405

View additional formats

• [Agarose](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Agarose/host/Goat/)
• [Alexa Fluor 350 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 350/host/Goat/)
• [Alexa Fluor 405 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 405/host/Goat/)
• [Alexa Fluor 430 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 430/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 514 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 514/host/Goat/)
• [Alexa Fluor 532 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 532/host/Goat/)
• [Alexa Fluor 546 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 546/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 568/host/Goat/)
• [Alexa Fluor 594 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 594/host/Goat/)
• [Alexa Fluor 633 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 633/host/Goat/)
• [Alexa Fluor 635 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 635/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 660 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 660/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 700 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 700/host/Goat/)
• [Alexa Fluor 750 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 750/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Alexa Fluor 790/host/Goat/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Goat/)
• [APC ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/APC/host/Goat/)
• [APC-Alexa Fluor 750](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/APC-Alexa Fluor 750/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Goat/)
• [Biotin-XX](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Biotin-XX/host/Goat/)
• [BODIPY FL](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/BODIPY FL/host/Goat/)
• [Cyanine3](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Cyanine3/host/Goat/)
• [Cyanine5 ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Cyanine5/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 350/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 680/host/Goat/)
• [DyLight 755](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Goat/)
• [DyLight 800](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Goat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Goat/)
• [Pacific Blue](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Pacific Blue/host/Goat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/PE/host/Goat/)
• [PE-Alexa Fluor 610](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/PE-Alexa Fluor 610/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine/host/Goat/)
• [Rhodamine Red-X](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine Red-X/host/Goat/)
• [Texas Red-X](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/Texas Red-X/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

400/423 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_2533208

Target

IgG

Cross Adsorption

Against human, bovine, horse, rabbit, swine, goat and rat serum proteins

Antibody Form

Whole Antibody

Product Specific Information

Product # 35500 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 35500 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by solid-phase adsorbed to ensure minimal cross-reactivity with human, bovine, horse, rabbit, swine, goat and rat serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 405 Amax= 400 nm; Emax= 420 nm. Mole Dye/Mole Protein Ratio is lot-dependent.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.