Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 405

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:20,000

Immunohistochemistry (IHC)

1:50-1:2,000

Immunocytochemistry (ICC/IF)

4 µg/mL

Flow Cytometry (Flow)

1:25 - 1:100

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Purified Mouse IgG, whole molecule

Conjugate

DyLight™ 405 DyLight™ 405 DyLight™ 405

View additional formats

• [Agarose](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Agarose/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 488-10 nm colloidal gold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488-10 nm colloidal gold/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 405 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 405/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/AP/host/Goat/)
• [bGal](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/bGal/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Biotin/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 350/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 680/host/Goat/)
• [DyLight 800 4X PEG](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 800 4X PEG/host/Goat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/HRP/host/Goat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/PE/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Rhodamine/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

400/423 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533209

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Product # 35501 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 35501 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 405 Amax= 400 nm; Emax= 420 nm. Mole Dye/Mole Protein Ratio is lot-dependent.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.