Thermo Fisher Scientific IL-4 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IL-4 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDYS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-4. Anti-Murine IL-4-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine IL-4 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-4 (500-P54) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-4.

Western Blot:
To detect Murine IL-4 by Western Blot, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Murine IL-4 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P54BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor. IL-4 has broad biological functions and shares receptor binding with IL-13, leading to overlapping cytokine activities. STAT6 mediates IL-4’s signal transduction. The IL-4 gene resides on chromosome 5q within a cytokine cluster including IL-3, IL-5, IL-13, and CSF2. IL-4 is closely located to IL-13 and IL-5, coordinated by long-range regulatory elements over a 120 kb region. IL-4 delta 2 is a known splice variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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