Thermo Fisher Scientific Phospho-beta Arrestin 1 (Ser412) Monoclonal Antibody (H.992.9)
Phospho-beta Arrestin 1 (Ser412) 단클론 항체로, 인간 및 설치류 시료에서 WB 및 IP에 적합합니다. beta-arrestin 2와 교차 반응하지 않으며, ERK1/2 매개 GPCR 신호 연구에 유용합니다. 액상 형태로 -20°C 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H.992.9 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser412 of human β-arrestin 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10980337 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with beta-arrestin 2.
Target Information
Beta Arrestin 1 is a member of a protein family widely expressed throughout the body, particularly abundant in the central nervous system. Acting as an adaptor or scaffold molecule, it is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of G protein-coupled receptors (GPCRs), such as the beta 2-adrenergic receptor.
Upon ligand binding and G protein interaction, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Cytosolic beta Arrestin 1 is phosphorylated by ERK1/2 on serine 412 in a negative feedback mechanism and binds to phosphorylated receptors at the plasma membrane. Subsequent dephosphorylation of serine 412 leads to GPCR internalization and activation of the Ras–Raf–ERK1/2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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