Thermo Fisher Scientific SRC Monoclonal Antibody (R.645.8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

R.645.8

Immunogen

Synthetic peptide corresponding to residues surrounding Tyr416 of human Src if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SRC,uniProtId:P12931-1,ncbiNodeId:9606,antigenRange:416,antigenLength:536,antigenImageFileName:MA5-15214_SRC_P12931-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15214_SRC_P12931-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.8 mg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 100µg/mL BSA, 0.15M NaCl, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10980540

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.