Thermo Fisher Scientific CD18 (LFA-1 beta) Monoclonal Antibody (M18/2), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• FITC
• PE
• Request custom conjugation

Product Specific Information

Description:
The M18/2 monoclonal antibody reacts with mouse CD18, a 90–95 kDa integrin beta 2 glycoprotein. CD18 forms heterodimers with CD11a (alpha L), CD11b (alpha M), and CD11c (alpha X) to generate LFA-1, Mac-1, and gp150/95. These complexes are broadly expressed on leukocytes, with higher expression on NK and T cells. CD18 plays a critical role in cellular adhesion and immune response through interactions with counter-receptors.

Applications Reported:
Reported for use in flow cytometric analysis and blocking of CD18 in functional studies.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. Recommended ≤0.5 µg/test (100 µL final volume). Cell number should be optimized (10⁵–10⁸ cells/test). Titrate for optimal assay performance.

Storage and Handling:
Use in a sterile environment. Store at 4°C.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90% (SDS-PAGE).

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).

Aggregation:
<10% (HPLC).

Target Information

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leukocytes. It forms heterodimers with CD11a–d to constitute leukocyte (β2) integrins:

• αLβ2 (CD11a/CD18, LFA-1)
• αMβ2 (CD11b/CD18, Mac-1, CR3)
• αXβ2 (CD11c/CD18)
• αDβ2 (CD11d/CD18)

These integrins mediate leukocyte adhesion and migration. Defects in CD18 cause leukocyte adhesion deficiency type I (LAD1). CD18 also mediates leukotoxin-induced cytolysis by Mannheimia haemolytica.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.