Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Functional Grade, eBioscience
Mouse CD19 인식하는 Rat IgG2a 단클론 항체로, Flow Cytometry 및 Immunohistochemistry(Frozen)에 적합. Functional Grade로 제조되어 세포 활성 실험에 사용 가능. 고순도(>90%) 및 저엔도톡신(<0.001 ng/µg) 보장. 4°C에서 보관, Wet ice 배송.
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- 16-0193-xxx (5개 옵션)
- 판매단위
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5개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Functional Grade, eBioscience
Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 126 publications |
| Functional Assay (Functional) | Assay-Dependent | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1D3 (1D3) |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865651 |
Available Formats
- Unconjugated
- Alexa Fluor 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor 450, 506, 660
- FITC
- NovaFluor Blue, Red, UV, Violet, Yellow Series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright Series (436–780)
- Custom conjugation available upon request
Product Specific Information
Description:
The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except plasma cells. CD19 forms a multimolecular complex with CD21, CD81, and MHC class II that associates with the BCR, mediating tyrosine phosphorylation, calcium flux, and B cell proliferation.
Applications Reported:
Used for flow cytometry and immunohistology of frozen tissue. Known to induce down-regulation of CD19 and alter B-1a/B-2 B cell proportions in mice.
Applications Tested:
Validated by flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Cell numbers may range from 10⁵–10⁸ per test. Antibody titration recommended for optimal performance.
Storage and Handling:
Use in a sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90% (SDS-PAGE).
Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).
Aggregation:
<10% (HPLC).
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on all peripheral B cells and some myeloid leukemia cells. It is the earliest and broadest B-cell restricted antigen and plays a key role in regulating normal and malignant B-cell proliferation. CD19 decreases the threshold for antigen receptor-dependent stimulation and acts as a signal-amplifying coreceptor for the BCR. Mutations cause hypogammaglobulinemia; overexpression leads to B-cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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