Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Functional Grade, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (421–786)
• eFluor 450, 506, 660
• FITC
• NovaFluor Blue, Red, UV, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright Series (436–780)
• Custom conjugation available upon request

Product Specific Information

Description:
The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except plasma cells. CD19 forms a multimolecular complex with CD21, CD81, and MHC class II that associates with the BCR, mediating tyrosine phosphorylation, calcium flux, and B cell proliferation.

Applications Reported:
Used for flow cytometry and immunohistology of frozen tissue. Known to induce down-regulation of CD19 and alter B-1a/B-2 B cell proportions in mice.

Applications Tested:
Validated by flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Cell numbers may range from 10⁵–10⁸ per test. Antibody titration recommended for optimal performance.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90% (SDS-PAGE).

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay).

Aggregation:
<10% (HPLC).

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on all peripheral B cells and some myeloid leukemia cells. It is the earliest and broadest B-cell restricted antigen and plays a key role in regulating normal and malignant B-cell proliferation. CD19 decreases the threshold for antigen receptor-dependent stimulation and acts as a signal-amplifying coreceptor for the BCR. Mutations cause hypogammaglobulinemia; overexpression leads to B-cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.