Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Functional Grade, eBioscience
Mouse CD11c 단백질을 인식하는 Armenian hamster 유래 단클론 항체로, Flow Cytometry 등 다양한 응용에 사용 가능. Functional Grade로 제조되어 세포 분석에 적합하며, 높은 순도(>90%)와 낮은 엔도톡신 수준(<0.001 ng/µg)을 보장. 4°C 보관, 1 mg/mL 농도.
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Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Functional Grade, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 2 publications |
| Immunohistochemistry (IHC) | - | View 20 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 6 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 127 publications |
| ELISA | - | View 1 publication |
| Control (Ctrl) | Assay-Dependent | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2865621 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605, 650, 711
- eFluor 450, 506, 615, 660
- FITC
- NovaFluor Blue 510, 610-30S, 660-40S, 690
- NovaFluor Yellow 570
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 780
- Request custom conjugation
Product Specific Information
Description:
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX). CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer, expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. It binds CD54, iC3b, and fibrinogen, mediating leukocyte adhesion. N418 binds CD11c on splenic dendritic cells and precipitates a 150/90 kDa heterodimer.
Applications Reported:
Used for flow cytometric analysis of mouse splenocytes.
Applications Tested:
Validated for flow cytometry at ≤1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Optimal titration recommended.
Endotoxin:
<0.001 ng/µg antibody (LAL assay).
Storage and Handling:
Use in sterile environment.
Filtration:
0.2 µm post-manufacturing filtered.
Purity:
90% (SDS-PAGE).
Aggregation:
<10% (HPLC).
Target Information
CD11 and CD18 form heterodimer adhesion molecules.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
- CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes.
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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