Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Functional Grade, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Functional Grade, eBioscience™

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506, 615, 660
• FITC
• NovaFluor Blue 510, 610-30S, 660-40S, 690
• NovaFluor Yellow 570
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

Description:
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX). CD11c associates with beta2 integrin to form the CD11c/CD18 heterodimer, expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. It binds CD54, iC3b, and fibrinogen, mediating leukocyte adhesion. N418 binds CD11c on splenic dendritic cells and precipitates a 150/90 kDa heterodimer.

Applications Reported:
Used for flow cytometric analysis of mouse splenocytes.

Applications Tested:
Validated for flow cytometry at ≤1 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Optimal titration recommended.

Endotoxin:
<0.001 ng/µg antibody (LAL assay).

Storage and Handling:
Use in sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90% (SDS-PAGE).

Aggregation:
<10% (HPLC).

Target Information

CD11 and CD18 form heterodimer adhesion molecules.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
• CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, granulocytes.
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.