Thermo Fisher Scientific CCL4 Monoclonal Antibody (24006)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Flow Cytometry (Flow)

0.25 µg per million cells

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

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Neutralization (Neu)

0.3-1.2 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

24006

Immunogen

Sf 21-derived recombinant human CCL4/MIP-1 beta Ala24-Asn92 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL4,uniProtId:P13236-1,ncbiNodeId:9606,antigenRange:24-92,antigenLength:92,antigenImageFileName:MA5-23713_CCL4_P13236-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23713_CCL4_P13236-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• Request custom conjugation

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609459

Product Specific Information

In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP-1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP-1 alpha, rmCCL4/MIP-1 beta, rhCXCL8/IL-8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.