Thermo Fisher Scientific NGF Monoclonal Antibody (25623)

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Neutralization (Neu)

0.1-0.4 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

25623

Immunogen

Mouse myeloma cell line NS0-derived recombinant human beta-NGF Ser122-Ala241 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NGF,uniProtId:P01138-1,ncbiNodeId:9606,antigenRange:122-241,antigenLength:241,antigenImageFileName:MA5-23717_NGF_P01138-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23717_NGF_P01138-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609810

Product Specific Information

In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) beta -NGF and recombinant rat (rr) beta ‑NGF is observed and less than 5% cross-reactivity with recombinant human (rh) BDNF, rhNT-3, and rhNT-4 is observed. This antibody neutralizes the bioactivity of rh beta -NGF, rm beta -NGF, and rr beta -NGF.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.