Thermo Fisher Scientific Phospho-RHOA (Ser188) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Product Specifications

Product Specific Information

This antibody detects a RhoA GST fusion protein when phosphorylated with PKA, but does not detect the unphosphorylated recombinant protein.

Target Information

RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal required for myosin contractile ring formation during cytokinesis and plays an essential role in cleavage furrow formation.
RhoA is required for apical junction formation of keratinocyte cell-cell adhesion and serves as a target for the yopT cysteine peptidase from Yersinia pestis and Yersinia pseudotuberculosis.
It stimulates PKN2 kinase activity and may activate PLCE1. RhoA is activated by ARHGEF2, promoting GDP–GTP exchange, and is essential for SPATA13-mediated regulation of cell migration and adhesion dynamics.
The MEMO1–RHOA–DIAPH1 signaling pathway is critical for ERBB2-dependent stabilization of microtubules at the cell cortex, controlling the localization of APC and CLASP2 to the cell membrane via regulation of GSK3B activity.
Membrane-bound APC allows localization of MACF1 to the cell membrane, necessary for microtubule capture and stabilization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.