Thermo Fisher Scientific Phospho-Profilin 1 (Tyr129) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-Profilin (Tyr129) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Tyr129 in human Profilin-1. This sequence is conserved in rat and mouse Profilin-1. The tyrosine site has some homology to the conserved site (Tyr129) in Profilin-2a and Profilin-2b, the site is not conserved in Profiin-3 and Profilin-4.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942981

Product Specific Information

This antibody was cross-adsorbed to unphosphorylated Profilin (Tyr-129) then affinity purified using Profilin (Tyr-129) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human umbilical vein endothelial cells (HUVEC) and jurkat cells treated with pervanadate. This reactivity is removed by alkaline phosphatase treatment.

Target Information

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.