Thermo Fisher Scientific Phospho-A-Raf/B-Raf/C-Raf (Ser471, Ser579, Ser432) Polyclonal Antibody

Applications

• Western Blot (WB): 1:1,000 dilution

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to phospho-C-Raf (Ser-338) peptide before affinity purification using phospho-C-Raf (Ser-471) peptide.
The purified antibody detects a band at 74 kDa corresponding to C-Raf in western blots of human Jurkat cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

Target Information

The Ras-Raf-MAP kinase signaling pathway regulates cell proliferation and differentiation.
The Raf kinase family includes A-Raf, B-Raf, and C-Raf, each containing three conserved regions (CR1–CR3):

• CR1: Ras-GTP-binding domain
• CR2: Negative regulatory serine residue (C-Raf Ser259 / B-Raf Ser365) that may bind 14-3-3 proteins
• CR3: Catalytic domain with phosphorylation sites for Raf-regulating enzymes

Activation of C-Raf involves phosphorylation at Ser-338, Tyr-341, and multiple catalytic domain sites.
In B-Raf, phosphorylation of Ser-446 may prime activation, while Ser-446 and/or Ser-447 are critical for biological activity during PC12 differentiation.
Ser-579 is required for growth factor activation and kinase activity.
Multiple phosphorylation sites within Rafs are important for regulation of their activity.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.