Thermo Fisher Scientific CREB Recombinant Rabbit Monoclonal Antibody (SA04-04)
CREB 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능. 인간, 마우스, 랫, 제브라피시 반응성. 고순도 Protein A 정제, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SA04-04 |
| Immunogen | Synthetic peptide within human CREB aa 250–290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2898841 |
Product Specific Information
This product is predicted to work in Immunoprecipitation.
Target Information
CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to various extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB depends on phosphorylation of serine 133. Phosphorylation occurs via p44/42 MAP kinase, p90RSK, p38 MAP kinase, and MSK1. Although CREB binds DNA independent of its phosphorylation state, only the phosphorylated form acts as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator, interacts with CREB near serine 133. CREB is involved in cellular processes such as circadian rhythm synchronization, adipocyte differentiation, and learning and memory. In humans, the gene is located on chromosome 2q. Diseases associated with CREB dysfunction include Alzheimer’s disease, histiocytoma, and soft tissue melanoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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