Thermo Fisher Scientific Phospho-MYPT1 (Ser509) Polyclonal Antibody
MYPT1 단백질의 Ser509 인산화 부위를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot에 적합하며 인간, 마우스, 랫트 시료에 반응. PBS/glycerol buffer에 보관되며 -20°C에서 안정적. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PPP1R12A (Accession O14974), corresponding to amino acid residues around phosphorylated Ser509 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816807 |
Product Specific Information
This antibody detects endogenous levels of MYPT1 only when phosphorylated at Ser509.
Target Information
Myosin phosphatase target subunit 1 (MYPT1), also known as the myosin-binding subunit of myosin phosphatase, is a regulatory subunit that controls actin–myosin interactions downstream of the small GTPase Rho. RhoA in its GTP-bound form activates Rho-kinase, which phosphorylates and inactivates MYPT1, leading to increased myosin light chain (MLC) phosphorylation and smooth muscle contraction. Overexpression or activation of RhoA enhances MBS and MLC phosphorylation. Several transcript variants encoding different isoforms of MYPT1 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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