Thermo Fisher Scientific Biotin Polyclonal Antibody, PE
Biotin을 인식하는 Rabbit Polyclonal Antibody로 PE 형광체가 결합되어 있습니다. Western blot, ICC/IF, Flow cytometry에 사용 가능하며 높은 특이성과 감도를 제공합니다. 연구용으로 적합하며 안정적인 보관 및 취급이 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:100–1:250 |
| Flow Cytometry (Flow) | 1:100–1:250 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- Restore with deionized water (or equivalent).
- Product is stable at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- Centrifuge product if not completely clear after standing at room temperature.
- Do not freeze after reconstitution.
- Store reagent in the dark.
- Use subdued lighting during handling and incubation of cells prior to analysis.
Assay Information:
Immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rabbit Serum, Biotin conjugated IgG, and Biotin conjugated Bovine Serum Albumin.
Target Information
Biotin is a water-soluble B complex vitamin (Vitamin B7). It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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