Thermo Fisher Scientific Biotin Polyclonal Antibody, Rhodamine
Western blot, ICC/IF, Dot blot에 사용 가능한 Rhodamine 결합 Biotin 폴리클로날 항체. Rabbit IgG 기반으로 높은 특이성과 감도를 제공. 생화학적 분석 및 형광 검출에 적합하며, 573/591 nm 파장에서 형광 특성. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,500 |
| Dot blot (DB) | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Rhodamine |
| Excitation / Emission Max | 573 / 591 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Biotin conjugated IgG, and Biotin conjugated Bovine Serum Albumin.
Target Information
Biotin is a water-soluble B complex vitamin (Vitamin B7). It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species.
Biotin is essential for the incorporation of carbon dioxide into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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