Thermo Fisher Scientific MEK2 C-Term Polyclonal Antibody, Biotin
Biotin으로 표지된 MEK2 C-말단 폴리클로날 항체로 인간, 생쥐, 랫트 반응성. Western blot 및 ELISA에 적합하며 MEK1과 비반응성. 친화 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공.
- 카탈로그번호
- 600-406-GQ2x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MEK2 C-Term Polyclonal Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This biotin-conjugated affinity purified antibody is directed against human MEK2 protein.
Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen.
This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a critical role in mitogen growth factor signal transduction.
MEK2 (MAPKK2) activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation at serine 218 and 222.
Threonine 292 of MEK1 is phosphorylated by ERK2, serving as a negative feedback mechanism.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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