Thermo Fisher Scientific VDAC Monoclonal Antibody (N152B/23), FITC
FITC로 표지된 VDAC1 단클론 항체로 Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트에 반응하며 교차 반응 없음. 단백질 G 정제, 1 mg/mL 농도, 4°C 암소 보관. 세포 대사 및 세포사 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N152B/23 |
| Immunogen | Fusion protein amino acids 1–283 (full-length) of human VDAC1 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.09 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932121 |
Additional Formats
- APC (Cat. No. MA5-45666)
- PerCP (Cat. No. MA5-45668)
- Custom conjugation available upon request
Product Specific Information
- Mouse: 98% identity (279/283 amino acids identical)
- Rat: 98% identity (279/283 amino acids identical)
60% identity with VDAC2 and VDAC3
- 1 µg/mL of MA5-45667 detects VDAC1 in 20 µg of rat brain lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP as secondary antibody
- Detects approximately 30 kDa protein
- Does not cross-react with VDAC2 or VDAC3 (KO validation)
- Formerly sold as clone S152B-23
Target Information
Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins located mainly in the mitochondrial outer membrane and also expressed in the plasma membrane. They play crucial roles in cellular metabolism and apoptosis regulation. VDAC enables exchange of metabolites such as ADP/ATP, succinate, and citrate between cytosol and mitochondria. VDAC1 in the plasma membrane may regulate apoptosis via redox activity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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