Thermo Fisher Scientific VDAC Monoclonal Antibody (N152B/23), PerCP
VDAC1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체입니다. Western blot, IHC, ICC/IF에 사용 가능하며 Human, Mouse, Rat에 반응합니다. PerCP 형광 표지로 482/675 nm에서 검출되며, 단백질 G로 정제된 액상 형태입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N152B/23 |
| Immunogen | Fusion protein amino acids 1–283 (full-length) of human VDAC1 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932122 |
Additional Formats
Product Specific Information
- Mouse: 98% identity (279/283 amino acids identical)
- Rat: 98% identity (279/283 amino acids identical)
60% identity with VDAC2 and VDAC3
- Detects approximately 30 kDa
- Does not cross-react with VDAC2 or VDAC3 (based on KO validation results)
- 1 µg/mL of MA5-45668 was sufficient for detection of VDAC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
- This antibody was formerly sold as clone S152B-23
Target Information
Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. They are found in the mitochondrial outer membrane and also expressed in the plasma membrane. At least three different VDAC genes have been identified in vertebrates. VDAC proteins play an essential role in cellular metabolism and in the early stages of apoptosis. VDAC constitutes a major pathway for metabolite exchange (ADP/ATP, succinate, citrate) between cytosol and mitochondria. VDAC1 in the plasma membrane is involved in apoptosis regulation via its redox activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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