Thermo Fisher Scientific Phospho-IRS1 (Ser794) Polyclonal Antibody
IRS1 단백질의 Ser794 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 인간, 생쥐, 랫트 반응성. 고순도 항원 친화 크로마토그래피 정제. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-IRS1 (Ser794) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:300 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser794 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608894 |
Product Specific Information
This antibody is predicted to react with human and mouse based on sequence homology.
Target Information
IRS-1 (Insulin Receptor Substrate 1) is a major substrate of the insulin receptor, phosphorylated in response to stimulation by insulin, IGF-1, and IL-4. It undergoes phosphorylation on serine, threonine, and tyrosine residues across various tissues. Casein kinase II mediates a portion of insulin-stimulated phosphorylation of IRS-1, with Thr502 and Ser99 identified as major phosphorylation sites. IRS-1 also contains three putative 14-3-3 binding sites (Ser270, Ser374, Ser641), with Ser270 located within the phosphotyrosine binding domain responsible for insulin receptor interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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