Thermo Fisher Scientific Phospho-MEK3 (Ser218) Polyclonal Antibody
Phospho-MEK3 (Ser218) 항체로, Western blot, IHC, ELISA에 사용 가능. 사람, 마우스, 랫에서 반응하며 rabbit polyclonal IgG 형식. 항원 친화 크로마토그래피로 정제, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:300 |
| ELISA | 1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human MEK-3 around the phosphorylation site of S189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608732 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase involved in cellular signal transduction pathways and is necessary for the expression of glucose transporter. It phosphorylates and activates MAPK14/p38-MAPK and can be activated by insulin. Expression of the RAS oncogene results in accumulation of the active form of this kinase, leading to constitutive activation of MAPK14 and oncogenic transformation of primary cells. Inhibition of this kinase is involved in the pathogenesis of Yersinia pseudotuberculosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported for MEK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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