Takara Reverse transcriptase for long RT-PCR: PrimeScript II

Overview

• Recombinant MMLV reverse transcriptase
• Includes accessory protein that inhibits mis-priming and mis-annealing events and promotes specificity
• Allows rapid cDNA synthesis at an RNA-friendly temperature (42°C)
• Provides higher yield during cDNA synthesis of long products compared to original PrimeScript RT and competitors’ enzymes
• Promotes efficient cDNA synthesis from highly structured RNA transcripts
• Delivers robust yield even after samples are pre-incubated on ice, allowing flexible workflows
• Suitable for construction of full-length cDNA libraries, 1st strand cDNA synthesis, preparation of cDNA probes, and RT-PCR

Storage

• Temperature: -20°C

Unit Definition

Quality Control Testing

PrimeScript II Reverse Transcriptase undergoes a 1st Strand cDNA Synthesis Test to assess performance for synthesis of a 13 kb cDNA product, as well as three different purity tests.
For more details, consult the product data sheet available under the Documents tab.

Additional Product Information

Refer to the product’s Certificate of Analysis for details about storage conditions, product components, and technical specifications.
The Kit Components List can be found under the Documents tab.