원본
Takara Methylation-dependent restriction enzyme McrBC
상품 한눈에 보기
DNA 메틸화 의존적 절단 효소로, 메틸화된 시토신(mC)을 포함한 DNA를 선택적으로 절단. 재조합 E. coli 단백질 기반, 37°C에서 반응. DNA 메틸화 분석 및 비메틸화 DNA 농축에 활용 가능. 구성품에는 McrBC Buffer, BSA, GTP 포함.
- 브랜드
- Takara
Takara · Takara Methylation-dependent restriction enzyme McrBC
Overview
- Source: Recombinant proteins expressed in E. coli
- Concentration: 1 unit/µl
- Supplied buffer: McrBC
- Reaction temperature: 37°C
- Substrate for unit definition: pBR322
Effect of DNA Methylation
This enzyme specifically cleaves DNA containing methyl cytosine (mC: 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu: A or G).
DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp.
Applications
- DNA methylation analysis (qAMP: quantitative analysis of DNA methylation using real-time PCR)
- Enrichment of unmethylated DNA (CHARM: Comprehensive high-throughput arrays for relative methylation)
Components
| Component | Volume |
|---|---|
| 10X McrBC Buffer | 500 μl |
| 0.1% BSA | 1 ml |
| 100 mM GTP (100×) | 50 μl |
Additional Product Information
Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
The Kit Components List and Certificates of Analysis are available under the Documents tab.
Takara 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.