Takara Methylation-dependent restriction enzyme McrBC

Overview

• Source: recombinant proteins expressed in E. coli
• Concentration: 1 unit /µl
• Supplied buffer: McrBC
• Reaction temperature: 37°C
• Substrate for unit definition: pBR322
• Effect of DNA methylation: This enzyme specifically cleaves DNA containing methyl cytosine (mC : 5-hydroxymethylcytosine, 5-methylcytosine, 4-methylcytosine) preceded by a purine nucleotide (Pu: A or G). DNA cleavage by McrBC requires at least two PumC sites within a distance of 40–2,000 bp.

More Information

Applications

• DNA methylation analysis (qAMP: quantitative analysis of DNA methylation using real-time PCR)
• Enrichment of unmethylated DNA (CHARM: Comprehensive high-throughput arrays for relative methylation)

Components

• 10X McrBC Buffer 500 μl
• 0.1% BSA 1 ml
• 100 mM GTP (100×) 50 μl

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.