Thermo Fisher Scientific CD169 (Siglec-1) Monoclonal Antibody (7-239), APC, eBioscience

Thermo Fisher Scientific CD169 (Siglec-1) Monoclonal Antibody (7-239), APC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 2 publications

Product Specifications

Product Specific Information

This 7-239 monoclonal antibody reacts with human CD169 (Siglec-1, Sialoadhesin), a type I transmembrane protein of the sialic acid binding Ig-like lectin (Siglec) family.
CD169 is expressed on macrophages and dendritic cells in human peripheral blood, lymph nodes, and spleen, and binds glycoproteins and glycolipids containing N-acetylneuraminyl α2-3-galactose.
Expression can be induced on CD14+ monocytes by interferon-α and interferon-γ, mediating HIV-1 infection and antigen presentation to invariant NK T cells.
This antibody inhibits erythrocyte binding to CD169-expressing cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of cultured human peripheral blood cells.
Use at 5 µL (0.06 µg) per test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test.
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Siglecs are membrane-bound lectins (immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions.
Family members include sialoadhesin, CD22, and CD33.
Sialoadhesin is a macrophage-specific adhesion molecule expressed on activated macrophages in chronic inflammation and tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Available Formats

• Alexa Fluor 488
• PE
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Request custom conjugation

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