Thermo Fisher Scientific CD161 Monoclonal Antibody (HP-3G10), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 3 publications

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• eFluor 450
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

This HP-3G10 monoclonal antibody reacts with CD161 (NKR-P1A), a member of the C-type lectin superfamily. CD161 is expressed on natural killer (NK) cells and various T cell subsets, including memory/effector CD4+ and CD8+ T cells, γδ T cells, and a subset of CD3+ thymocytes. It is also found on Th17 CD4+ T cells. The receptor’s function is not fully understood, but studies suggest a stimulatory role. Lectin-like transcript-1 (LLT1), also known as osteoclast inhibitory lectin, has been identified as its ligand.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on human peripheral blood cells
• Recommended Use: 5 µL (0.25 µg) per test (100 µL total volume, 10⁵–10⁸ cells/test)
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

KLRB1 (CD161) is a type II membrane protein expressed on almost all NK cells and a subset of CD3⁺ T cells. It forms a homodimeric cell surface protein (40–44 kDa) and plays an inhibitory role on NK cells. Activation of KLRB1 stimulates acid sphingomyelinase/SMPD1 and increases intracellular ceramide, as well as activating AKT1/PKB and RPS6KA1/RSK1 kinases, promoting T cell proliferation by anti-CD3.

KLRB1 binds to:

• Gal-α(1,3)Gal and N-acetyllactosamine epitopes
• CLEC2D/LLT1 ligand, inhibiting NK cell-mediated cytotoxicity and IFN-γ secretion

The protein contains an extracellular C-type lectin domain, a transmembrane domain, and a cytoplasmic domain.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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