Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), APC, eBioscience
인산화된 ZAP70/Syk (Tyr319, Tyr352)을 검출하는 APC 결합 단클론 항체로 인간 및 마우스 시료에 반응. 유세포 분석용으로 최적화되어 있으며, 5 µL/test 농도로 사용. TCR 및 BCR 신호전달 연구에 적합하며, 고품질 친화 크로마토그래피로 정제됨.
- 판매단위
- pk
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Thermo Fisher Scientific Phospho-ZAP70/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), APC, eBioscience
Applications
- Flow Cytometry (Intracellular staining)
Tested Dilution: 5 µL (0.03 µg)/test
Publications: View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), APC |
| Class | Monoclonal |
| Type | Antibody |
| Clone | n3kobu5 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573268 |
Product Specific Information
This n3kobu5 monoclonal antibody recognizes human and mouse ZAP-70 and SYK when phosphorylated on Y319 and Y352, respectively.
ZAP-70 and SYK are members of the SYK protein tyrosine kinase family activated by Src family kinases.
Phosphorylation of ZAP-70 Y319 by Lck is essential for TCR-dependent signaling, while SYK Y352 phosphorylation by Fyn/Lyn is critical for BCR signaling and B cell development.
Specificity was confirmed by ELISA, flow cytometry, and western blotting.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated for intracellular staining of stimulated human peripheral blood cells. Recommended usage: 5 µL (0.03 µg)/test in 100 µL final volume. Cell count may vary from 10⁵ to 10⁸ cells/test.
Staining Protocols
Protocol A: Two-step (cytoplasmic proteins) – optimal for surface and intracellular detection.
Protocol B: One-step (nuclear proteins) – recommended for transcription factor staining.
Protocol C: Two-step (Fixation/Methanol) – best for phospho-specific signaling discrimination.
Detailed protocols available in BestProtocols® - Staining Intracellular Antigens for Flow Cytometry.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase of the Syk family, expressed in T cells and NK cells, essential for TCR activation.
Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319.
Phosphorylation of 315 and 319 enhances signaling, while 292 attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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