Thermo Fisher Scientific Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), APC, eBioscience
Phospho-S6 (Ser235, Ser236) 단백질을 인식하는 cupk43k 단클론 항체로, 인간 및 마우스 시료에 사용 가능. APC 형광체 결합으로 유세포분석(Flow Cytometry)에 최적화. 세포 성장 및 신호전달 연구용으로 적합하며, 4°C 암소 보관 권장.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.06 µg)/test
- Publications: View 4 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | cupk43k |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573269 |
Additional Formats Available:
Product Specific Information
This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (human) and serine 236 (mouse).
Ribosomal protein S6 is a component of the 40S ribosomal subunit and is phosphorylated following stimulation by growth factors, tumor-promoting agents, or mitogens. Phosphorylation by p70S6K and PKDCD upregulates translation of proteins involved in cell cycle entry, while dephosphorylation occurs during growth arrest.
- Specificity: Confirmed by western blotting
- Reported Applications: Intracellular staining followed by flow cytometric analysis
- Tested Applications: Pre-titrated and validated for intracellular staining and flow cytometry of stimulated human peripheral blood cells
- Recommended Usage: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
Protocol Recommendations
- Protocol A: Two-step (cytoplasmic) – optimal for detecting surface and intracellular proteins
- Protocol B: One-step (nuclear) – for transcription factors with surface/phospho-proteins
- Protocol C: Two-step (Fixation/Methanol) – best for phospho-specific discrimination, limited surface staining
Detailed protocols are available in the BestProtocols® guide.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Ribosomes catalyze protein synthesis and consist of a small 40S and a large 60S subunit. The S6 protein, part of the 40S subunit, belongs to the S6E family and serves as a major substrate for ribosomal protein kinases. It contains multiple serine residues phosphorylated by various kinases in response to stimuli such as growth factors and mitogens. Dephosphorylation occurs during growth arrest. S6 may regulate cell growth and proliferation through selective mRNA translation. Multiple processed pseudogenes exist throughout the genome.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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