Thermo Fisher Scientific Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.06 µg)/test
• Publications: View 4 publications

Product Specifications

Additional Formats Available:

• Unconjugated
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (human) and serine 236 (mouse).
Ribosomal protein S6 is a component of the 40S ribosomal subunit and is phosphorylated following stimulation by growth factors, tumor-promoting agents, or mitogens. Phosphorylation by p70S6K and PKDCD upregulates translation of proteins involved in cell cycle entry, while dephosphorylation occurs during growth arrest.

• Specificity: Confirmed by western blotting
• Reported Applications: Intracellular staining followed by flow cytometric analysis
• Tested Applications: Pre-titrated and validated for intracellular staining and flow cytometry of stimulated human peripheral blood cells
• Recommended Usage: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Protocol Recommendations

• Protocol A: Two-step (cytoplasmic) – optimal for detecting surface and intracellular proteins
• Protocol B: One-step (nuclear) – for transcription factors with surface/phospho-proteins
• Protocol C: Two-step (Fixation/Methanol) – best for phospho-specific discrimination, limited surface staining

Detailed protocols are available in the BestProtocols® guide.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ribosomes catalyze protein synthesis and consist of a small 40S and a large 60S subunit. The S6 protein, part of the 40S subunit, belongs to the S6E family and serves as a major substrate for ribosomal protein kinases. It contains multiple serine residues phosphorylated by various kinases in response to stimuli such as growth factors and mitogens. Dephosphorylation occurs during growth arrest. S6 may regulate cell growth and proliferation through selective mRNA translation. Multiple processed pseudogenes exist throughout the genome.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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