Thermo Fisher Scientific DNMT3A/DNA Methyltransferase 3 Alpha Monoclonal Antibody (PCRP-DNMT3A-1E2)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

DNA methylation at cytosine residues is crucial for regulation of gene expression, genomic imprinting, and mammalian development.
Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of genomic DNA is associated with cancer development.
Three families of mammalian DNA methyltransferases have been identified: Dnmt1, Dnmt2, and Dnmt3.

• Dnmt1: Constitutively expressed in proliferating cells; its inactivation causes global demethylation and embryonic lethality.
• Dnmt2: Expressed at low levels in adult tissues; inactivation does not affect DNA methylation.
• Dnmt3 family (Dnmt3a, Dnmt3b): Strongly expressed in embryonic stem (ES) cells; expression decreases during differentiation and is low in adult tissues.
• Mutations in Dnmt3b are associated with ICF syndrome (Immunodeficiency, Centromeric instability, and Facial anomalies).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.