Thermo Fisher Scientific TNNT1 Polyclonal Antibody
Rabbit polyclonal antibody against human TNNT1, validated for Western blot and ELISA. Recognizes recombinant TNNT1 (aa 1–262). Supplied as liquid, affinity purified, 1.62 mg/mL in PBS with 50% glycerol. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–262 of human TNNT1 (NP_001119604.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.62 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804443 |
Product Specific Information
Immunogen sequence:
MSDTEEQEYE EEQPEEEAAE EEEEAPEEPE PVAEPEEERP KPSRPVVPPL IPPKIPEGER VDFDDIHRKR MEKDLLELQT LIDVHFEQRK KEEEELVALK ERIERRRSER AEQQRFRTEK ERERQAKLAE EKMRKEEEEA KKRAEDDAKK KKVLSNMGAH FGGYLVKAEQ KRGKRQTGRE MKVRILSERK KPLDIDYMGE EQLREKAQEL SDWIHQLESE KFDLMAKLKQ QKYEINVLYN RISHAQKFRK GAGKGRVGGR WK
Positive Samples: HT-1080, A-549, SW480
Target Information
The TNNT1 gene encodes a protein that is a subunit of troponin, a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to intracellular calcium fluctuations. It consists of three subunits:
- Troponin C: binds calcium
- Troponin T: binds tropomyosin
- Troponin I: inhibitory subunit
TNNT1 encodes the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5 (Amish nemaline myopathy), a neuromuscular disorder characterized by muscle weakness and rod-shaped inclusions in skeletal muscle fibers, typically fatal in infancy due to respiratory insufficiency. Multiple transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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