Thermo Fisher Scientific TCR DO11.10 Monoclonal Antibody (KJ1-26 (KJ126, KJI-26, KJI26)), Biotin, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 6 publications

Product Specifications

Available Formats

• APC
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The KJ1-26 anti-idiotypic monoclonal antibody binds to the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on other T cell hybridomas. The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-A^d^ major histocompatibility molecules.
DO11.10 and KJ1-26 have been instrumental in elucidating key mechanisms of T cell immunity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on DO11.10 T cell hybridoma at ≤0.25 µg/test
• Definition of a test: Antibody amount staining a cell sample in 100 µL final volume
• Recommended: Titrate antibody for optimal assay performance

Target Information

TCR DO11 (DO11.10) is a receptor found on lymphocytes of DO11.10 transgenic mice and T-cell receptors of BALB/c-derived DO11.10 and DO11.10.24 T cell hybridomas.
The DO11.10 TCR recognizes the C-terminal ovalbumin peptide (OVA 323–339) in the context of I-Ad molecules.
Antibodies against DO11.10 are widely used to study T cell activation, development, and immune function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.