Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• APC
• APC-eFluor 780
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Red 700
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains, recognizing the majority of the TCR V alpha 2 subfamily in mice carrying the a, b, and c haplotypes. It also cross-reacts with TCR V delta 8 products due to high sequence homology.

Applications Reported:
Flow cytometric analysis and immunoprecipitation.

Applications Tested:
Tested by flow cytometric analysis of mouse lymph node cells. Recommended usage ≤ 0.125 µg per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Pre-block Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (Product #14-0161-85).
Antibody titration is recommended for optimal performance.
Filtered through 0.2 µm post-manufacturing.

Target Information

T cell receptors (TCRs) enable discrimination between foreign and self-peptides presented by MHC class II molecules, a key mechanism in adaptive immunity.
Recognition of self-peptides is linked to autoimmune diseases, while mutant self-peptides are associated with tumors.
Superantigen engagement of TCRs can cause toxic shock syndrome.
Autoantibodies to V beta segments of TCRs have been found in rheumatoid arthritis and systemic lupus erythematosus, potentially modulating TH1-mediated inflammation.
Human T cells mainly express TCR alpha-beta with CD4 or CD8, while gamma-delta T cells, found in epithelia, show less diversity and distinct antigen recognition, contributing to antitumor and immunoregulatory activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.