Thermo Fisher Scientific Phospho-PRAS40 (Ser183) Polyclonal Antibody
PRAS40 단백질의 Ser183 인산화 상태를 특이적으로 검출하는 폴리클로날 항체. Western blot 및 IHC(P) 실험에 적합하며, 인간·마우스·랫트 시료 반응. 고순도 정제, 액상 형태로 -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AKT1S1 (Accession Q96B36), corresponding to amino acid residues around phosphorylated Ser183 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816109 |
Product Specific Information
This antibody detects endogenous levels of PRAS40 only when phosphorylated at Ser183.
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after various cell death signals.
AKT1S1 (PRAS40) is a proline-rich substrate of the kinase AKT1 and plays a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia.
It is also a substrate and potential regulator of mTOR, a serine/threonine kinase regulating cell growth and cycle, and a negative regulator of autophagy.
Treatment with insulin-like growth factor-1 (IGF1) can induce phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: PA5-104634_PRAS40_Q96B36-1_Rabbit_PDP.jpeg)
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