Thermo Fisher Scientific CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), eBioscience
마우스 CD11a(LFA-1α)에 특이적인 Rat IgG2a 단일클론 항체로, 면역조직화학, 유세포분석, 면역침강 등에 적합합니다. LFA-1 관련 세포접착 및 면역반응 연구용으로 사용되며, 다양한 형광 및 기능 등급 포맷으로 제공됩니다.
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- 14-0111-8x (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | 3 publications |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 15 publications |
| Immunoprecipitation (IP) | Assay-Dependent | 1 publication |
| Neutralization (Neu) | Assay-Dependent | - |
| Functional Assay | Assay-Dependent | - |
| Inhibition Assays | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M17/4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467105 |
Available Formats
- APC
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 610-70S, Blue 660-120S
- NovaFluor Red 685, Red 700, Red 755
- NovaFluor Yellow 610, Yellow 660, Yellow 700, Yellow 755
- PE, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436, 600, 702
- Request custom conjugation available
Product Specific Information
The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L, also known as lymphocyte function-associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), acts as an adhesion molecule involved in lymphocyte and granulocyte function. CD11a is expressed by all leukocytes and interacts with ligands CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3).
Applications Reported:
The M17/4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been used for blocking CD11a function (functional assays should use Functional Grade purified M17/4, Product #16-0111).
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes, recommended ≤0.5 µg per test (100 µL final volume). Optimal titration is recommended for best assay performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD11a (LFA-1 alpha, ITGAL) together with CD18 forms LFA-1 (alphaLbeta2 integrin), crucial for leukocyte adhesion and costimulatory signaling. CD11a interacts with ICAMs 1–3 and transitions between low- and high-affinity conformations upon cell activation. It plays roles in immune cell migration, adhesion, differentiation, and T-cell effector function. Blocking CD11a is a therapeutic strategy in inflammatory diseases; e.g., Efalizumab (Raptiva) targets CD11a to modulate T-cell migration. Simvastatin also binds CD11a, influencing immune modulation. Two transcript variants exist; dysfunctions are linked to benign cephalic histiocytosis and leukocyte adhesion deficiency.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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