Thermo Fisher Scientific Hemoglobin alpha Polyclonal Antibody
인간 Hemoglobin alpha 단백질을 인식하는 Chicken Polyclonal Antibody입니다. Western blot 및 ELISA에 적합하며, 15 kDa의 Hemoglobin alpha를 검출합니다. 합성 펩타이드를 면역원으로 제작되었으며, PBS에 0.02% sodium azide를 포함한 액상 형태로 제공됩니다.
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- PA19527
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide mixture based on the human hemoglobin alpha protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgY fraction |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2114460 |
Product Specific Information
PA1-9527 detects Hemoglobin alpha in human samples.
PA1-9527 has been successfully used in Western blot and ELISA procedures.
By Western blot, PA1-9527 detects a 15 kDa protein representing Hemoglobin alpha.
The immunogen is a synthetic peptide based on the human hemoglobin alpha protein.
Target Information
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci:
5′- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3′.
The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5′ untranslated regions and introns, but differ significantly over the 3′ untranslated regions.
Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of total hemoglobin. Alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin.
Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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