Thermo Fisher Scientific Phospho-TRAP220 (Thr1457) Polyclonal Antibody
TRAP220/MED1 단백질의 Thr1457 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간 및 생쥐 시료 반응성. 고순도 정제 및 안정한 PBS/glycerol 용액 형태로 제공.
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- PA5104998
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MED1 (Accession Q15648), corresponding to amino acid residues around phosphorylated Thr1457. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2816471 |
Product Specific Information
This antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Threonine 1457.
Target Information
TRAP220/MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge conveying information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It is recruited to promoters through direct interactions with regulatory proteins and serves as a scaffold for assembling a functional preinitiation complex with RNA polymerase II and general transcription factors.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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