Thermo Fisher Scientific BrdU Monoclonal Antibody (A9C7)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:200

-

Flow Cytometry (Flow)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A9C7

Immunogen

BrdU-OVA

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3090201

Product Specific Information

Positive control: BrdU treated mouse embryo tissue, BrdU treated Hela, BrdU treated NIH/3T3, EdU treated mouse embryo tissue, EdU treated Hela, EdU treated NIH/3T3.

Subcellular Location: Nucleus.

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.