Thermo Fisher Scientific WDR41 Monoclonal Antibody (A9C10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A9C10

Immunogen

Recombinant protein within human aa 231-430/459. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:WDR41,uniProtId:Q9HAD4-1,ncbiNodeId:9606,antigenRange:231-430,antigenLength:459,antigenImageFileName:MA5-50619_WDR41_Q9HAD4-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50619_WDR41_Q9HAD4-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3090541

Product Specific Information

Positive control: Daudi cell lysate, SiHa cell lysate, rat kidney tissue, human testis tissue, human colon tissue, Hela.

Predicted band size: 52 kDa

Subcellular Location: Cytoplasm.

Target Information

Non-catalytic component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. The C9orf72-SMCR8 complex promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation. As part of the C9orf72-SMCR8 complex, stimulates RAB8A and RAB11A GTPase activity in vitro, however WDR42 is shown not be an essential complex component for this function. The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ULK1/ATG1 kinase complex and inhibiting its protein kinase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.