Thermo Fisher Scientific Phospho-Tau (Thr522) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Tau only when phosphorylated at Thr522, a site historically referenced as Thr205.

Target Information

Tau is a neuronal microtubule-associated protein predominantly localized on axons. It promotes tubulin polymerization and stabilizes microtubules.

• The C-terminus binds axonal microtubules.
• The N-terminus binds neural plasma membrane components, acting as a linker between both structures.

Axonal polarity is determined by Tau (MAPT) localization in the neuronal cell body domain defined by the centrosome.
Short isoforms provide cytoskeletal plasticity, while longer isoforms contribute to stabilization.

In its hyper-phosphorylated form, Tau constitutes the major component of paired helical filaments (PHF), the building blocks of neurofibrillary lesions in Alzheimer’s disease (AD) brains.
Hyper-phosphorylation impairs Tau’s microtubule-binding function, destabilizing microtubules and leading to neuronal degeneration.

Tau phosphorylation is mediated by several kinases, including:

• GSK-3β
• Protein kinase A (PKA)
• Cyclin-dependent kinase 5 (CDK5)
• Casein kinase II

Hyper-phosphorylated Tau is also found in neurofibrillary lesions associated with other central nervous system disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.