Thermo Fisher Scientific Phospho-Tau (Thr548) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Tau only when phosphorylated at Thr548, which site is historically referenced as Thr231.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, functioning as a linker between both structures. Axonal polarity is determined by TAU/MAPT localization in the neuronal cell body domain defined by the centrosome. Short isoforms provide cytoskeletal plasticity, whereas longer isoforms contribute to stabilization.

In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building blocks of neurofibrillary lesions in Alzheimer’s disease (AD) brains. Hyper-phosphorylation impairs Tau’s microtubule-binding function, leading to microtubule destabilization and neuronal degeneration. Tau phosphorylation is mediated by several serine/threonine kinases including GSK-3β, PKA, cdk5, and casein kinase II. Hyper-phosphorylated Tau is also found in neurofibrillary lesions associated with other CNS disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.