Thermo Fisher Scientific ALK (Anaplastic Lymphoma Kinase)/CD246 Recombinant Rabbit Monoclonal Antibody (ALK1/7008R)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Immunoelectrophoresis (IE)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ALK1/7008R

Immunogen

Recombinant human ALK protein fragment corresponding to the cytoplasmic domain. The antigen corresponds to amino acid range 1058-1620 of the target protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ALK,uniProtId:Q9UM73-1,ncbiNodeId:9606,antigenRange:1058-1620,antigenLength:1620,antigenImageFileName:238-RBM10-P1_ALK_Q9UM73-1_Rabbit.svg,antigenImageFileNamePDP:238-RBM10-P1_ALK_Q9UM73-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Antibody is stable for 24 months.

Positive Control: Human Anaplastic Large Cell Lymphoma (ALCL) tissue. Cellular Localization: Cytoplasm. Nuclear.

Specificity Comments: Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase of the insulin receptor superfamily. ALK is typically expressed at low levels in regions of the developing central and peripheral nervous system.ALK may be activated in cancer through multiple mechanisms. The most common mechanism is through formation of a fusion protein from chromosomal translocations, as in the case of anaplastic large cell lymphoma (ALCL) and inflammatory myofibroblastic tumors. ALK may also be amplified through mutation, as in neuroblastomas. Various solid tumors, such as non-small cell lung carcinoma (NSCLC) and brain cancers were also found to aberrantly express ALK. ALK staining is present within both the nucleus and cytoplasm, and are positive in about 60% of ALCL. ALK protein expression by tumor cells is an independent prognostic factor that predicts a favorable outcome.

Target Information

Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) belongs to the insulin receptor superfamily. It is vital for brain development. Mutations, rearrangements, and amplifications in the ALK gene have been found in tumors, including anaplastic large cell lymphoma, neurblastoma, and non-small cell lung cancer. Chromosomal rearrangement is the most common genetic alteration. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3half of ALK, derived from chromosome 2, is fused to the 5 portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.