Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), APC, eBioscience
마우스 CD25(IL-2Rα)를 인식하는 PC61.5 클론의 APC 결합 단클론 항체로, 유세포분석(Flow Cytometry)에 최적화됨. 0.125 µg/test 권장. 651/660 nm 형광 특성. 4°C 암소 보관, 동결 금지. 연구용 전용 제품.
- 카탈로그번호
- 17-0251-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | 191 publications |
| Functional Assay (Functional) | - | 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469365 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC-eFluor 780
- Biotin
- Brilliant UV 563, 661, 737, 805
- Brilliant Violet 421, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue 585, 610-70S, 660-120S, 760
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. CD25 binds IL-2 with low affinity alone, but forms a high-affinity receptor when associated with CD122 (IL-2Rβ) and CD132 (common γ chain). Binding of IL-2 to both receptor classes is inhibited by the PC61.5 antibody. CD25 is involved in lymphocyte differentiation and activation/proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Optimal dilution should be determined empirically (10⁵–10⁸ cells/test).
Fluorescence: Excitation 633–647 nm; Emission 660 nm; Laser: Red.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD25 (IL2 receptor alpha chain / IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation. The IL2RA gene encodes the α-chain of the heterotrimeric IL-2 receptor complex (with IL2RB and IL2RG).
- Homodimeric IL2RA: low-affinity receptor
- Homodimeric IL2RB: medium-affinity receptor
- Heterotrimeric IL2RA/IL2RB/IL2RG: high-affinity receptor
IL2 is primarily produced by mature T cells and functions as a growth and differentiation factor, as well as a regulator of cell death. It stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte-macrophage colony formation. Mutations in IL2RA are linked to interleukin-2 receptor alpha deficiency and immune dysregulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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