Thermo Fisher Scientific ATG9A Monoclonal Antibody (14F2 8B1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:100

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

14F2 8B1

Immunogen

peptide sequence HPEPVPEEGSEDELPPQVHK of human ATG9A C-terminus if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATG9A,uniProtId:Q7Z3C6-1,ncbiNodeId:9606,antigenRange:819-839,antigenLength:839,antigenImageFileName:MA1-149_ATG9A_Q7Z3C6-1_Armenian_hamster.svg,antigenImageFileNamePDP:MA1-149_ATG9A_Q7Z3C6-1_Armenian_hamster_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536859

Product Specific Information

MA1-149 was produced in Armenian hamster and detects ATG9 in human, rat and mouse samples. MA1-149 has been successfully used in Western Blot, Immunofluorescence, and ELISA procedures. Western Blot analysis with MA1-149 shows the detection of a double band at ~85-95 kDa in human ATG9 overerexpression lysates. MA1-149 also detects additional unknown bands at ~40 and ~60 kDa. In Immunofluorescence applications, MA1-149 shows accumulation and redestribution of ATG9 in response to starvation-induced autophagosome assembly.

Target Information

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.