Thermo Fisher Scientific RAP1A Monoclonal Antibody (1D4-1A3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Immunoprecipitation (IP)

2 µg

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

1D4-1A3

Immunogen

Recombinant full-length human Rap1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAP1A,uniProtId:P62834-1,ncbiNodeId:9606,antigenRange:1-184,antigenLength:184,antigenImageFileName:MA1-147_RAP1A_P62834-1_House_mouse.svg,antigenImageFileNamePDP:MA1-147_RAP1A_P62834-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536858

Product Specific Information

Western blot analysis of MA1-147 detects an ~21kDa protein. In IHC (paraffin) applications, this antibody has only shown reactivity with human samples.

Target Information

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.