Thermo Fisher Scientific SMAD5 Recombinant Rabbit Monoclonal Antibody (SI17-06)
SMAD5 단백질을 인식하는 재조합 토끼 단일클론 항체로, 높은 특이성과 민감도를 제공. WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. HEK293 세포에서 발현되며, 동물 유래 성분이 없는 포뮬레이션. 장기 보관 시 -20°C에서 안정적.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SI17-06 |
| Immunogen | Synthetic peptide within Human SMAD5 (aa 231–282) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809359 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select the optimal production candidates.
Advantages:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
SMADs are intracellular proteins essential for signaling pathways of serine/threonine kinase receptors in the transforming growth factor beta (TGF-β) superfamily.
They are classified as:
- R-SMADs: SMAD1, 2, 5, 8, 9
- Co-SMAD: SMAD4
- I-SMADs: SMAD6, 7
SMAD1, 5, 8, and 9 share high sequence homology. SMAD8 and SMAD9 are often used interchangeably in literature.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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