Thermo Fisher Scientific Phospho-Histone H10 (Ser1) Polyclonal Antibody
Histone H4 Ser1 인산화 부위를 인식하는 rabbit polyclonal antibody로, WB, IHC, ICC, ChIP, Dot blot 등에 사용 가능. Human, Mouse, Rat 반응성. 친화 크로마토그래피로 정제되었으며, -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:10,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H4 [p Ser1] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 1 of human Histone H4. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.89 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate (pH 7.2), 30% glycerol, 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Histone H4 [p Ser1] reacts with human Histone H4. A BLAST analysis suggests cross-reactivity with human, mouse, and rat, and it is predicted to react with most mammalian species. Cross-reactivity with Histone H4 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction. The histone H4 gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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