Thermo Fisher Scientific H4K8ac Polyclonal Antibody
Histone H4K8ac에 특이적인 rabbit polyclonal antibody로 다양한 종에서 반응. Western blot, IHC, ICC, ChIP 등 다중 응용 가능. 액상 형태로 제공되며 -20°C 보관. Affinity chromatography로 정제된 고품질 연구용 항체.
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Thermo Fisher Scientific H4K8ac Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2 µg/mL |
| Immunohistochemistry (IHC) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000–1:2,000 |
| ChIP assay (ChIP) | 2–5 µg/1×10^6 cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H4 [ac Lys8] affinity purified antibody prepared from rabbit serum immunized with a synthetic acetylated peptide surrounding Lysine 8 of human Histone H4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Histone H4 [ac Lys8] reacts with human Histone H4. A BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans. Cross-reactivity with Histone H4 from other sources has not been determined.
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in compaction of chromatin into higher order structures.
This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. The gene is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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