Thermo Fisher Scientific H3K56me3 Polyclonal Antibody
H3K56me3 폴리클로날 항체로 히스톤 H3의 트리메틸화된 Lys56을 인식합니다. WB, IHC, ICC/IF, ChIP, Dot blot 등 다양한 응용에 적합합니다. 인간, 생쥐, C. elegans 등 여러 종에 반응하며, 고순도 친화 크로마토그래피 정제 제품입니다.
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Thermo Fisher Scientific H3K56me3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:40 |
| Immunocytochemistry (ICC/IF) | 1:40 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Trimethyl Lys56] affinity purified antibody prepared from whole rabbit serum immunized with a synthetic trimethylated peptide surrounding Lysine 56 of human Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.71 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Histone H3 [Trimethyl Lys56] reacts with human Histone H3. BLAST analysis suggests cross-reactivity with human, mouse, and C. elegans. Predicted to react with rat, chicken, Xenopus, Drosophila, and plant species based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it binds DNA to form nucleosomes. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones, including methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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