Thermo Fisher Scientific H3K79me3 Polyclonal Antibody
H3K79me3에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, ChIP, Dot blot 등 다양한 응용 가능. 인간 및 여러 종에 반응하며, 고순도 Affinity Chromatography로 정제된 액상 형태. 연구용으로만 사용.
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Thermo Fisher Scientific H3K79me3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (IHC) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Trimethyl Lys79] affinity purified antibody prepared from rabbit serum immunized with synthetic trimethylated peptide surrounding Lys79 of human Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
Anti-Histone H3 [Trimethyl Lys79] reacts with human Histone H3.
BLAST analysis suggests cross-reactivity with human, mouse, and C. elegans.
Predicted to react with rat, chicken, Xenopus, Drosophila, and plant species based on 100% sequence homology.
Cross-reactivity with other sources has not been determined.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells.
Together with other core histones, it forms the nucleosome structure.
Histones regulate transcription, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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