Thermo Fisher Scientific RANKL (soluble) Monoclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

ELISA (ELISA)

1.0-2.0 µg/mL

-

Dot blot (DB)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human sRANK Ligand

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_3074164

Product Specific Information

AA Sequence of recombinant protein: MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID.

Preparation: Produced in BALB/c x ICR F1 mice immunized with highly pure Recombinant Human sRANK Ligand. Anti-Human sRANK Ligand-specific antibody was purified from cell culture by Protein G affinity chromatography.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.